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ABSTRACT Introduction: The data on the pattern of primary hematologic malignancies in Bahrain is sparse, although previously published studies suggested rising trends in their incidence. This study aimed to compare with regional and world data and identify any changing trends. Methods: A retrospective cross-sectional chart analysis study was done on all cases of primary hematologic malignancies of bone marrow origin of Bahraini nationals presenting during the 10-year period from January 2005 to December 2014 at the sole oncology referral center in Bahrain during the study period. Results: In a total of 272 cases, the primary hematologic malignancies in decreasing order of frequency with respective median ages at diagnosis were: acute myeloid leukemia (AML; 26.1%, 39 years), acute lymphoblastic leukemia (ALL; 22.8%, 9 years), multiple myeloma (MM, 16.2%, 57 years), chronic myeloid leukemia (CML, 14%, 39.5 years), myelodysplastic syndromes (MDS; 12.5%, 56 years) and chronic lymphocytic leukemia (CLL; 5.5%, 65 years). The overall crude annual incidence rate of these malignancies was 4.8/105 population. Age-specific incidence rates were found to increase dramatically with age, except for ALL, for which it peaked in the pediatric age group. The age-standardized incidence rates (ASIRs) per 105 per year were 1.47 (AML), 1.13 (MM), 0.93 (ALL), 0.85 (MDS), 0.81 (CML) and 0.44 (CLL). Conclusion: The pattern of primary hematologic malignancies in Bahrain shows unique features that distinguish it from trends reported in Eastern and Western world populations.
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Objective: Pax-7 and Myo-D regulate satellite cells' activation and differentiation, thus muscle regeneration following damage. This research aimed to investigate the effect of Thymoquinone (TQ) on skeletal muscle regeneration following 7,12-dimethylbenz-(a)-anthracene (DMBA)-induced injury in the hamster buccal pouch via immunohistochemical assessment of Pax-7 and Myo-D expression. Material and Methods: 65 male golden Syrian hamsters were divided into 3 groups: Group 1: (n=5) received no treatment. Group 2: (n=20) served as a positive control. The left buccal pouches were painted with the carcinogen 3/week/ 6weeks. Group 3: (n=40) were subdivided into two equal sub-groups as follows: Group 3a: (n=20) were given one i.p. TQ injection. Group 3b: (n=20) were given two i.p. TQ injections. Five animals from each group (2 and 3) were euthanized at 24, 48 hrs, one, and two weeks after the last injection. A blood sample (2 ml) was withdrawn for assessment of TNF-α levels in serum. Serial sections of the pouches were examined histologically (H&E), and immunohistochemically (IHC) for the detection of Pax-7 and Myo-D proteins. Results: double i.p injections of TQ resulted in a significant elevation in the level of TNF-α from the second-day post-injection with a progressive formation of the muscle fibers (MFs) and mononuclear cells (MNCs) around the deeper blood vessels. At 14 days, no statistically significant difference was found between this group and group '2', while the difference remained significant compared to groups '1' and '3a'. The muscle fibers were more mature and compact. IHC results showed positive expression of the perivascular mononuclear cells (MNCs) to both Pax-7 and Myo-D with positive reactivity of the peripheral nuclei of muscle fibers to Pax-7 compared to the negative reaction in the positive control group. Conclusion: early and two TQ injections had a promising effect on the induction of striated muscle regeneration, mainly by non-myogenic stem cells (AU)
Objetivo: Pax-7 e Myo-D regulam a ativação e diferenciação de células satélites durante a regeneração muscular pós-trauma. Assim, objetivamos investigar o efeito da timoquinona (TQ) na regeneração muscular esquelética após injúria causada por 7,12 dimetilbenzantraceno (DMBA) em bolsa jugal de hamsters, através da análise imuno-histoquímica de Pax-7 e Myo-D. Material e Métodos: 65 hamsters-sírios machos foram divididos em 3 grupos: Grupo 1: (n=5) controle negativo, sem tratamento. Grupo 2: (n=20) controle positivo. A bolsa jugal do lado esquerdo recebeu aplicação do DMBA por 3 e 6 semanas. Grupo 3: (n=40) receberam aplicação de DMBA e foram então subdivididos em: Grupo 3a: (n=20) que recebeu 1 injeção intraperitoneal (ip) de TQ e Grupo 3b: (n=20) que recebeu duas injeções ip de TQ. Cinco animais dos grupos 2 e 3 foram eutanasiados em 24 horas, 48 horas, 7 dias e 14 dias após a administração de DMBA e da última injeção de TQ. Amostras de sangue (2 ml) foram coletadas para avaliação dos níveis séricos de TNF-α. Cortes seriados da bolsa jugal dos animais foram analisados histologicamente (H&E), e através de imunohistoquimica (IHC) para avaliação das proteínas Pax-7 e Myo-D. Resultados: duas injeções ip de TQ aumentaram os níveis séricos TNF-α à partir do segundo dia pós-administração com formação progressiva de fibras musculares (MFs) e células mononucleares (MNCs) ao redor dos vasos sanguíneos. No dia 14, não houve diferença estatística entre o grupo 3b e o grupo 2, enquanto a diferença permaneceu entre o grupo 1 e 3a. As MFs apresentavam-se mais maduras e compactas. A IHC mostrou expressão de Pax-7 e Myo-D nas MNCs ao redor dos vasos, e houve expressão nuclear de Pax-7 nas MFs no grupo 2. Conclusão: ambos regimes de administração do TQ, 1 ou 2 aplicações ip, apresentaram efeito promissor na indução da regeneração muscular esquelética, principalmente nas células não-miogênicas.(AU)
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Animals , Immunohistochemistry , 9,10-Dimethyl-1,2-benzanthracene , PAX7 Transcription FactorABSTRACT
Objective: To explore the efficacy of earthworm's coelomic fluid against gentamicin-induced hepatic and renal toxicity in rats. Methods: The animals were divided randomly into three groups (n = 6 per group): control, gentamicin, and Allolobophora caliginosa coelomic fluid-treated groups. Toxicity was established after injection of gentamicin daily for 8 days at a dose of 100 mg/kg. Aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total proteins, albumin, creatinine, urea, uric acid, malondialdehyde, glutathione, catalase and histopathology of tissues were investigated in the study. Results: Allolobophora caliginosa coelomic fluid significantly decreased urea, creatinine, uric acid, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and malondialdehyde levels while significantly increasing levels of total proteins, albumin, glutathione and catalase. The histopathological investigation showed partial restoration of renal and hepatic architecture. Conclusions: This study shows the potency of Allolobophora caliginosa coelomic fluid in improving the biochemical and histopathological changes induced by gentamicin in the liver and kidney of the rats.
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Background: Diarrhea is second major killer disease for children less than 5 year of age; and maternal lack of sound knowledge and related correct practices inn this regard conflicts with WHO treatment guidelines for diarrhea. Objective: 1) to assess the level of maternal knowledge and practices regarding prevention and management of diarrhea at home; in children below five year of age 2) to identify association between maternal knowledge and practices regarding prevention and care of diarrhea at home. Materials & Methods: It was a cross sectional study conducted in three tertiary level hospitals of Lahore selected randomly. Study population was mothers having children less than 5 year of age coming to pediatric Outdoor in these hospitals. Calculated sample size was 296; and non-probability purposive sampling technique used to recruit the sample. Data was collected from 300 respondents; study instrument was KAP questionnaire adopted and modified and data was taken upon the variables of knowledge and practice. Data analysis plan was: descriptive statistics for all variables, Bivariate analysis upon knowledge and practice scores, Pearson Correlation upon the two variable scores. Results: 61.7% respondents had good knowledge score, whereas 32% and 6.3% had average and poor score respectively. 39.3% were found to have good practice score, whereas 54% and 6.7% were found to have average and poor practice level score. Highly significant association (P- 0.000) was found between knowledge and practice levels; and Pearson correlation of 43.8% was found between the two variables. Conclusion: Health education communication should be conducted by health care workers so as to give the right knowledge about ORS intake and its preparation; and adoption of correct dietary practices by the mothers during diarrheal episode of the child.
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Background and Objectives: Helicobacter pylori [H. pylori] infection is cause of several gastrointestinal diseases in humans. Virulence genes of H. pylori are associated with severity of disease and vary geographically. The aim of present study was to detect H. pylori in formalin-fixed paraffin-embedded [FFPE] tissues and further investigate prevalence of babA2, cagA, iceA1, iceA2, vacA s1/s2 and vacA m1/m2 genotypes in H. pylori from gastric cancer [GC] and gastric ulcer [GU] patients' biopsy samples
Methods: We used FFPE tissues of 35 GC and 10 GU patients' biopsy samples. Using Polymerase Chain Reaction [PCR], detection of H. pylori strain was performed by using specific primers targeting 16S rRNA and ureC encodes for phosphoglucosamine mutase genes. We have identified different virulence genes of H. pylori by PCR
Results: Of all the 45 samples tested, 20 GC and all 10 GU samples were positive for identification of H. pylori using specific genes [16S rRNA and ureC]. The prevalence of babA2 [100%] was significantly higher in GC as compared to GU [40%] samples. The rate of virulence genes vacAs1 was higher in both GU 8 [80%] and GC [100%]
Conclusions: Our study finds that vacAs1am1 and babA2 are most prominent genotypes and may play role in development of Gastric cancer
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Objective:To compare the efficacy of titrated oral mis oprostol solution with intravenous oxytocin for augmentation of labour in term primigravidae in active phase of labour with inadequate uterine contractions
Methodology:This randomized control trial was conducted in the department of Gynaecology and Obstetrics, Pakistan Institute of Medical Sciences, Islamabad, over a period of six months from 14-April to 13-October, 2014.A total of 760 [two groups of 380 each] primigravidae, between age 20-39 years, who had completed 37-42 gestational weeks by dates, or by ultrasound scan, with regular contractions and an effaced cervix dilated between 3-4 cm, and who later developed inadequate uterine contractions during the first stage of labour were included in the study. Group A received titrated oral misoprostol solution 200ìg tablet dissolved in 200ml tap water and 20ml [20ìg/hour] and group-B was given Intravenous oxytocin [10 units in 1000cc Hartman's solution at 8 drops/minute, doubling every 30 minutes up to a maximum of 64 drops/min for 2 hours]
Results: Mean [+/-SD] age of the patients was 26.4+/-4.4 and 26.6+/-4.6 years in group-A and B respectively. In group-A, 322 patients [84.7%] and in group-B 326 patients [85.8%] were delivered vaginally. Mean [+/-SD] augmentation to delivery interval was 293.82+/-99.36 and 311.65+/-106.73 minutes in group-A and B respectively. Mean [+/-SD] gestational age in group-A was 38.82+/-1.32 and in group-B 38.83+/-1.09 week. Caesarean section was performed in rest of the patients in both groups. There was no significant association between mode of delivery in both groups [P-value= 0.682]
Conclusion:Labour augmentation with titrated oral misoprostol or intravenous oxytocin resulted in about similar rates of vaginal delivery